Septins

Content: 
Methods in Cell Biology Page i  
Series Editors Page ii  
Front Matter Page iii  
Copyright Page iv  
Contributors Pages xi-xiv  
Preface Page xv Amy S. Gladfelter 
Chapter 1 - The nonopisthokont septins: How many there are, how little we know about them, and how we might learn moreOriginal Research Article Pages 1-19 M. Onishi, J.R. Pringle 
Chapter 2 - Preparing recombinant yeast septins and their analysis by electron microscopyOriginal Research Article Pages 21-34 A. Bertin, E. Nogales 
Chapter 3 - A FRET-based method for monitoring septin polymerization and binding of septin-associated proteinsOriginal Research Article Pages 35-56 E.A. Booth, J. Thorner 
Chapter 4 - In vitro reconstitution of septin assemblies on supported lipid bilayersOriginal Research Article Pages 57-71 A.A. Bridges, A.S. Gladfelter 
Chapter 5 - Visualization of in vivo septin ultrastructures by platinum replica electron microscopyOriginal Research Article Pages 73-97 K. Ong, T. Svitkina, E. Bi 
Chapter 6 - Assays for genetic dissection of septin filament assembly in yeast, from de novo folding through polymerizationOriginal Research Article Pages 99-116 M.A. McMurray 
Chapter 7 - Investigation of septins using infection by bacterial pathogensOriginal Research Article Pages 117-134 S. Krokowski, S. Mostowy 
Chapter 8 - In vivo analysis of septin heteropolymer rods and higher-order structures in filamentous fungiOriginal Research Article Pages 135-141 A. Smith, M. Momany 
Chapter 9 - Live cell imaging of septin dynamics in Ustilago maydisOriginal Research Article Pages 143-159 S. Baumann, S. Zander, S. Weidtkamp-Peters, M. Feldbrügge 
Chapter 10 - Ashbya gossypii as a model system to study septin organization by single-molecule localization microscopyOriginal Research Article Pages 161-182 C. Kaplan, C. Yu, H. Ewers 
Chapter 11 - Visualizing septins in early Drosophila embryosOriginal Research Article Pages 183-198 M. Mavrakis 
Chapter 12 - Purification of recombinant human and Drosophila septin hexamers for TIRF assays of actin–septin filament assemblyOriginal Research Article Pages 199-220 M. Mavrakis, F.-C. Tsai, G.H. Koenderink 
Chapter 13 - Investigation of septin biology in vivo using zebrafishOriginal Research Article Pages 221-241 A. Willis, M. Mazon-Moya, S. Mostowy 
Chapter 14 - Fluorescence microscopy of actin- and microtubule-associated septins in mammalian cellsOriginal Research Article Pages 243-268 E.T. Spiliotis, E.P. Karasmanis, L. Dolat 
Chapter 15 - Immunofluorescent staining of septins in primary ciliaOriginal Research Article Pages 269-283 M.S. Kim, C.D. Froese, H. Xie, W.S. Trimble 
Chapter 16 - Methods for immunoblot detection and electron microscopic localization of septin subunits in mammalian nervous systemsOriginal Research Article Pages 285-294 L.K. Parajuli, N. Ageta-Ishihara, H. Ageta, Y. Fukazawa, M. Kinoshita 
Chapter 17 - Visualizing septin and cell dynamics in mammalian brain slicesOriginal Research Article Pages 295-309 H. Ito, R. Morishita, H. Tabata, K. Nagata 
Chapter 18 - Small molecule perturbations of septinsOriginal Research Article Pages 311-319 L.R. Heasley, M.A. McMurray 
Chapter 19 - Septin crystallization for structural analysisOriginal Research Article Pages 321-338 N.F. Valadares, R.C. Garratt 
Index Pages 339-352  
Volumes in Series Pages 353-365